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KMID : 1195620080010020075
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Clinical and Experimental Otorhinolaryngology
2008 Volume.1 No. 2 p.75 ~ p.79
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Modulators of Ion Transport in Nasal Polyps:An in situ Measurement of Short-Circuit Current
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Lee Jun-Ho
Rhee Chae-Seo
Kim Dae-Woo
Lee Chul-Hee
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Abstract
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Objectives: To examine possible modulators of the ion transport through the apical membrane of the nasal polyps.
Methods: The study was conducted using the freshly-excised nasal polyps from the patients with chronic sinusitis. A voltage-sensitive vibrating probe technique was introduced to monitor the short-circuit current across the apical membrane of the polyp at 37℃.
Results: In the presence of amiloride, Adenosine 5’-triphosphate induced 4,4’-Diisothiocyanatostilbene-2,2’-disulfonic acidsensitive chloride current. Uridine 5’-diphosphate was less potent than Uridine 5’-triphosphate, and adenosine increased chloride secretion, which was blocked by the antagonist, 8-(p-sulfophenyl) theophylline on adenosine receptor. Based on the pharmacologic profiles, multiple purinergic receptors, including P2Y2, P2Y6, and P1 receptors, were functionally expressed. However, P2X receptor agonists ( , -methyleneadenosine 5’-triphosphate and 2’- & 3’-O-[4-benzoyl-benzoyl] adenosine 5’-triphosphate), Cystic fibrosis conductance regulator (CFTR) activator (genistein), nitric oxide substrate (L-arginine), and nitric oxide donor (sodium nitroprusside) had no significant effect on the short circuit current.
Conclusion: Among tested drugs, P2Y receptor agonists were major modulators of ion transport in nasal polyps in situ.
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KEYWORD
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Nasal polyps, CFTR, Purinergic receptors, Genistein, Nitric oxide, Vibrating Probe
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